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Published on: May 22, 2018
Comparative mapping in the pig: localization of 214 expressed sequence tags
Susanna Cirera1, Claus B Jørgensen, Milena Sawera
1Department of Animal Science and Animal Health, Division of Genetics, The Royal Veterinary and Agricultural University, Groennegaardsvej 3, 1870 Frederiksberg C, Denmark.
Summary
Researchers mapped 214 expressed sequence tags (ESTs) to the porcine gene map using advanced techniques. This significantly advances comparative genomics between pigs and humans, identifying potential orthologous genes.
Area of Science:
- Genomics
- Comparative Genomics
- Bioinformatics
Background:
- Developing a comprehensive porcine gene map is crucial for understanding swine biology and genetics.
- Comparative mapping with human genes aids in identifying conserved genomic regions and functions.
Purpose of the Study:
- To assign expressed sequence tags (ESTs) to the porcine gene map.
- To contribute to the comparative map between human and pig genomes.
- To identify potential orthologous genes between species.
Main Methods:
- Somatic cell hybrid mapping, radiation hybrid mapping, and fluorescence in situ hybridization (FISH) were employed.
- ESTs were derived from a porcine small intestine cDNA library based on sequence identity to human genes.
- Primer pairs were designed for mapping, primarily in the 3' untranslated region (UTR).
Main Results:
- A total of 214 ESTs were targeted for mapping onto the porcine gene map.
- 58.6% of the ESTs were successfully mapped using the described methods.
- 191 mapped ESTs aligned with the human comparative map, suggesting true orthologs; 23 provided novel comparative data.
Conclusions:
- The study significantly contributes to the porcine gene map and comparative genomics.
- The findings support the identification of orthologous genes between humans and pigs.
- The generated data provides a foundation for future genetic and genomic studies in swine.
