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Updated: Aug 14, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
[Screening for factor V Leiden mutation in women using combined hormonal contraceptives or hormone replacement
D Cibula1, V Unzeitig, J Zivný
1Gynekologicko-porodnická klinika 1. LF UK a VFN v Praze. david.cibula@oil.cz
Objective:
Analysis of the issue of screening for Factor V Leiden mutation (FVL) in users of combined oral contraceptives (COC) or hormone replacement therapy (HRT).
Design:
Review article.
Setting:
Department of Obstetrics and Gynecology, Charles University and General Faculty Hospital, Prague.
Methods:
Review of published facts, analysis of possibilities to screen all users or to test risk groups in the current situation in the Czech Republic.
Conclusion:
1) Screening for FVL in all COC or HRT users is not recommended mostly for economical reasons. 2) Testing for FVL is indicated in women with a positive family history of thromboembolic disease and in obese women, if they consider COC or HRT despite our recommendation or in case there is a medical indication. 3) Deep venous thrombosis or pulmonary embolism are absolute contraindications of COC or HRT regardless of the presence of FVL.
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