Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.0K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.0K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.0K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

3.0K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.0K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.1K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.8K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.8K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Replacing Ecologically Risky Trifluoroacetic Acid and Acetonitrile With Methanesulfonic Acid and Dimethyl Carbonate in High-Performance Liquid Chromatography Analyses of Small Molecule Drugs.

Journal of separation science·2026
Same author

AbDist: a lightweight, distance-based model for antibody affinity prediction as an interpretable benchmark for machine learning models.

mAbs·2026
Same author

Improvement of Peak Integration in Capillary Electrophoresis: Reference Data Set No. 1.

Electrophoresis·2026
Same author

Peak Integration of Electropherograms in GMP and Research Labs: Navigating Increased Scrutiny Amid Data Integrity Audits and Inspections.

Electrophoresis·2025
Same author

Electrokinetic Chromatography-based Micro Methods for Separation and Physiochemical Characterization of Very Hydrophobic Pharmaceuticals.

Journal of separation science·2025
Same author

Evaluation of a cIEF Fractionation Workflow for Offline MS Analysis of Charge Variants of the Monoclonal Antibody Matuzumab.

Electrophoresis·2025

Related Experiment Video

Updated: May 2, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

32.4K

Capillary electrophoresis-a high performance analytical separation technique.

Hermann Wätzig1, Stefan Günter

  • 1Institute for Pharmaceutical Chemistry, Technical University Braunschweig, Braunschweig, Germany. h.waetzig@tu-bs.de

Clinical Chemistry and Laboratory Medicine
|July 26, 2003
PubMed
Summary

Capillary electrophoresis (CE) offers high selectivity and precision for pharmaceutical and clinical analysis. This technique requires minimal sample preparation, enabling rapid, cost-effective quality control.

More Related Videos

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

10.6K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

8.9K

Related Experiment Videos

Last Updated: May 2, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

32.4K
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

10.6K
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

8.9K

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Capillary electrophoresis (CE) is a preferred technique in pharmaceutical quality control and clinical chemistry.
  • CE offers high selectivity, precision comparable to liquid chromatography (LC), and high sample throughput due to short analysis times.
  • Minimal sample pre-treatment is required, allowing direct injection of samples like urine and blood plasma.

Purpose of the Study:

  • To summarize the basic principles of CE.
  • To describe general strategies for method development in CE.
  • To provide guidance on achieving selective, efficient, precise, fast, sensitive, and validated CE methods.

Main Methods:

  • Discussion of general strategies for CE method development.
  • Compilation of standard buffer recipes, surfactants for micellar electrokinetic chromatography (MEKC), chiral selectors, and buffer additives.
  • Strategies for handling complex matrices and aspects of method validation.

Main Results:

  • CE provides high selectivity and precision with short analysis times, leading to high sample throughput.
  • Direct sample injection from complex matrices like urine and blood plasma is feasible with minimal pre-treatment.
  • Various components for method optimization, including buffers, surfactants, and chiral selectors, are detailed.

Conclusions:

  • CE is a powerful, cost-effective technique for pharmaceutical and clinical analysis.
  • Effective method development strategies and optimization components are crucial for successful CE applications.
  • CE instruments can also perform other techniques like capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), and capillary electrochromatography (CEC).