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Updated: Aug 11, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Determination of the artificial sweetener Sucralose by capillary electrophoresis
1European Commission, DG Joint Research Centre, Institute for Health and Consumer Protection, Food Products Unit, I-21020 Ispra, Italy. joerg.stroka@irmm.jrc.be
Abstract:
The artificial intense sweetener 1,6-dichloro-1,6-dideoxy-beta-D-fructofuranosyl-4-chloro-4-deoxy-alpha-D-galactopyranose (Sucralose) was determined by capillary electrophoresis with indirect ultraviolet absorption in a 3,5-dinitrobenzoic acid buffer at pH 12.1. The method allowed determination of Sucralose in low-calorie soft drinks, without any sample clean-up over a linear range of 42-1000 mg x l(-1) (r=0.9991). The limits of detection and determination were 28 and 42 mg x l(-1), respectively, and the repeatability for a mean concentration of 100 mg x l(-1) was 4.2% for the signal area and 3.6% for the migration time, which were deemed satisfactory for use in food control.
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