Related Experiment Video
Updated: Sep 21, 2026
Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
Published on: January 28, 2015
PBSC harvests individually optimized by using pre-collection CD34(+) values and on-line flow cytometric analysis of
A Svensson1, P Engervall, T Söderstrom
1Department of Clinical Immunology and Transfusion Medicine, Karolinska Hospital, Stockholm Sweden.
Background:
We have investigated how the amount of blood processed during collection of PBSC affects the yield of CD34 cells.
Methods:
We first established a method of significantly increasing the enrichment of mononuclear cells (MNC), CD34(+) cells and granulocyte-macrophage progenitors (GM-CFU) by on-line flow cytometric (FCM) analysis of the leukocyte populations in the collect line. A total of 166 PBSC collections from 94 patients, were devided into five groups according to the blood volume processed: < 12 L of processed blood, 12-13L, 13-14L, 14-15L and > 15L.
Results:
When the yield of CD34(+) cells war compared between these groups, a positive correlation was seen (r=0.97) between the processed blood volume and the yield, expressed as a ratio between total number of CD34(+) cells in the harvest and the CD34(+) cell concentration in blood. This correlation can be used to estimate the volume that must be processed to exceed a specific target number of CD34(+) cells. The implications of these results on the need for one, two or more leukapheresis procedures in order to collect a sufficient amount of stem and progenitor cells for a given patient are discussed, i n relation to clinical logistics and the benefits for the patients.
Discussion:
PBSC harvest can be improved by individually-adjusted leukapheresis according to on-line FCM analysis and pre-harvest levels of CD34(+) cells and the processed blood volume can be used to predict the CD34(+) yield.
