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Purification and characterization of chitinase from Alcaligenes xylosoxydans
Rajiv Vaidya1, Satarupa Roy, Simone Macmil
1Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, Vadodara-390 002, Gujarat, India.
Abstract:
Extracellular chitinase from Alcaligenes xylosoxydans was purified to electrophoretic homogeneity using affinity and gel filtration chromatography. The molecular mass of chitinase was estimated to be 45 kDa and 44 kDa by SDS-PAGE and gel-filtration, respectively. The enzyme was optimally active at 50 degrees C (over 30 min) and pH 5. Activity staining after PAGE showed a single band. The Km for chitin was 3 g l-1. Cu2+ and Na+ at 5 mM inhibited chitinase activity to 25% while Ca2+, Mg2+ and Ba2+ had no effect at the same concentration. The purified enzyme degraded mycelia of Aspergillus niger.