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Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis
Werner Hummel1, Bettina Riebel
1Institut für Enzymtechnologie der Heinrich-Heine-Universität, Forschungszentrum Jülich, Wilhelm-Johnen-Str., D-52426 Jülich, Germany. w.hummel@fz-juelich.de
Abstract:
A new NADH oxidase, useful for the regeneration of NAD+, was isolated and characterized from Lactobacillus brevis. In crude extracts the activity was from 10-15 U mg(-1). After purification by four chromatographic steps, an activity of 116 U mg(-1) was obtained with 14% yield. Highest activity was from pH 5.5-7 and at 40 degrees C. The enzyme requires dithiothreitol to prevent oxidative deactivation. The Km value for NADH was 24 microM.
