Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methylated DNA labels for marking objects.

Lindsey J Cook1, Jonathan P L Cox

  • 1Department of Chemistry, University of Bath, Bath BA2 7AY UK.

Biotechnology Letters
|July 29, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Odorant transport in a hagfish.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2024
Same author

Olfactory flow in the sea catfish, Ariopsis felis (L.): Origin, regulation, and resampling.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2021
Same author

The functional nasal anatomy of the pike, Esox lucius L.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2020
Same author

Olfactory flow in the sturgeon is externally driven.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2019
Same author

Motion-driven flow in an unusual piscine nasal region.

Zoology (Jena, Germany)·2016
Same author

Complex flow in the nasal region of guitarfishes.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2016
Same journal

Insight of Pichia kudriavzevii for single cell protein production and fermentation into ethanol using Thai banana as an alternative feedstock.

Biotechnology letters·2026
Same journal

CitM mediates hydroxycitrate (HCA) uptake: insights from heterologous expression in Escherichia coli, Bacillus subtilis mutant analysis and membrane topology prediction.

Biotechnology letters·2026
Same journal

Foliar and root absorption of LDH nanosheets loaded with dsRNAs efficiently silenced the expression of whitefly Bemisia tabaci Laccase 1 and Vitellogenin Receptor genes for their effective control.

Biotechnology letters·2026
Same journal

Morfo-anatomical insights into the germination and protocorm growth of the endangered Vanilla lindmaniana (Orchidaceae).

Biotechnology letters·2026
Same journal

Biosynthetic glycosylation as a critical structural determinant of antifungal activity in venturicidin A.

Biotechnology letters·2026
Same journal

Scaling up biomass production of Vishniacozyma victoriae and semi-commercial testing for post-harvest control of pome fruit disease.

Biotechnology letters·2026
See all related articles

Researchers enhanced digital object labeling by using DNA methyltransferases to create polymorphic DNA templates. This method significantly increases the number of unique, invisible DNA labels detectable on surfaces like paper.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Forensic Science

Background:

  • Digital object labeling is crucial for tracking and authentication.
  • Previous methods for DNA-based object labeling had limitations in scalability and robustness.

Purpose of the Study:

  • To enhance the capacity of DNA-based digital object labeling.
  • To develop a method for generating a larger number of unique DNA labels.
  • To assess the stability and detectability of these DNA labels on physical objects.

Main Methods:

  • Utilized DNA methyltransferases (M.HaeIII, M.AluI) to create polymorphic DNA templates with varying methylation states.
  • Created mixtures of methylated and unmethylated DNA templates to serve as 'invisible' labels.
  • Applied these DNA mixtures to label paper surfaces.

Related Experiment Videos

  • Eluted DNA mixtures from paper and analyzed the methylation status using restriction endonuclease digestion, polymerase chain reaction (PCR), and agarose gel electrophoresis.

    • Main Results:

    • Successfully increased the number of distinguishable DNA labels by employing polymorphic DNA templates.
    • Demonstrated the ability to accurately read and decode the 'invisible' DNA labels from paper.
    • Confirmed the stability of a DNA label, which remained readable after two months on paper.

    Conclusions:

    • Polymorphic DNA templates generated by DNA methyltransferases significantly expand the multiplexing capability of DNA-based object labeling.
    • This enhanced method offers a robust and scalable approach for invisible, high-capacity digital labeling of objects.
    • The technique shows promise for applications requiring secure and long-lasting object identification.