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Attenuated total reflectance Fourier transform mid-infrared spectroscopic quantification of sorbitol and sorbose
Sue Macauley-Patrick1, S Alison Arnold, Brendan McCarthy
1Strathclyde Fermentation Centre, Department of Bioscience, University of Strathclyde, 204 George St., Glasgow G1 1XW, UK.
Biotechnology Letters
|July 29, 2003
Abstract:
Mid-infrared spectroscopy (MIRS) was used to simultaneously detect and predict concentrations of D-sorbitol and L-sorbose during a Gluconobacter suboxydans biotransformation. Quantitative models for both these compounds were developed for the entire time-course of the process and validated externally using samples not included in the original modelling exercise, giving standard errors of prediction of 3.29 and 3.3% for sorbitol and sorbose, respectively, and a correlation coefficient close to 1.