Search research articles

Search research articles

Related Experiment Videos

Recombinant Sendai virus vectors for activated T lymphocytes.

S Okano¹, Y Yonemitsu, S Nagata

¹Division of Pathophygiological and Experimental Pathology, Department of Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

|July 29, 2003

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Strongyloides ratti: implication of mast cell-mediated expulsion through FcepsilonRI-independent mechanisms.

Parasite (Paris, France)·2009

Same author

Strongyloides ratti: the role of interleukin-5 in protection against tissue migrating larvae and intestinal adult worms.

Journal of helminthology·2003

Same author

IL-7 prevents both caspase-dependent and -independent pathways that lead to the spontaneous apoptosis of i-IEL.

Cellular immunology·2001

Same author

Role of Fas-Fas ligand interaction in donor-specific transfusion-induced tolerance to H-Y antigen.

Transplantation proceedings·2001

Same author

The effect of interleukin-4 on the induction of intestinal mast cells and chronological cytokine profiles during intestinal nematode Strongyloides ratti infection.

Parasitology research·2001

Same author

Dietary conjugated linoleic acid increases immunoglobulin productivity of Sprague-Dawley rat spleen lymphocytes.

Bioscience, biotechnology, and biochemistry·2000

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Sendai virus vectors (SeV) efficiently deliver genes into activated T-lymphocytes for immunological disorder treatment. This novel gene therapy approach overcomes low efficiency seen with other methods, offering a promising clinical alternative.

Area of Science:

Immunology
Gene Therapy
Virology

Background:

T-lymphocyte gene therapy shows promise for immunological disorders.
Current limitations include low gene transfer efficiency, hindering therapeutic potential.

Purpose of the Study:

To evaluate the efficiency and specificity of a recombinant Sendai virus vector (SeV) for T-lymphocyte gene transfer.
To explore SeV as a potential solution for improving gene therapy efficacy in T cells.

Main Methods:

Utilized a recombinant Sendai virus vector (SeV) carrying the enhanced green fluorescent protein (EGFP) gene.
Tested transduction efficiency in activated and naive murine and human T cells.
Investigated SeV's tropism and potential mechanisms for selective gene transfer.

Related Experiment Videos

Main Results:

SeV efficiently transduced and expressed foreign genes in activated T cells, but not naive T cells.
Gene transfer occurred without complex procedures like centrifugation or specific reagents.
Transduction was selective for antigen-activated T cells, avoiding bystander activation effects.
Receptor saturation studies suggested SeV entry is restricted in naive T cells.

Conclusions:

Recombinant SeV vectors demonstrate high efficiency and specificity for gene transfer into activated T cells.
SeV offers a potentially superior alternative to traditional viral vectors like retroviruses in T-cell gene therapy.
This SeV vector system presents a promising advancement for clinical applications in T-cell-directed gene therapy.