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Updated: Jan 4, 2026
In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
Published on: May 4, 2017
Expression of recombinant human ICOS and in vitro characterization of its bioactivity on B lymphocytes
Zhong-Bin Deng1, Chang-Ming Lu, Wei-Da Huang
1Biotechnology Research Institute, Soochow University, Suzhou 215007, China. smbxuegz@public1.sz.js.cn
Abstract:
Inducible costimulator (ICOS) is a novel costimulatory molecule expressed in activated T cell and has critical regulation effect on special immune response. In this study, the cDNA encoding human ICOS was cloned from activated tonsil cells via RT-PCR, and was expressed in E. coli on pET28 expression vector. The recombinant ICOS protein expressed from E. coli showed a molecular weight of 14 kD on SDS-polyacrylamide gel electrophoresis and was further confirmed by Western blot. In presence of IL-10, the purified rhICOS significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM), and enhanced the secretion of IgG from B cells.
