Ultraviolet spectrometry of serum triglycerides by a totally enzymic method adapted to a centrifugal analyzer

We described a micromethod for determining serum triglycerides (triacylglycerols) with the centrifugal analyzer. This technique is based on the procedure of Bublitz and Kennedy [J. Biol. Chem. 211, 951 (1954)]. The enzymic hydrolysis requires 10 min at 30 degrees C. Twenty-six serum triglyceride assays can be done in about 30 min. Concentration and absorbance are linearly related up to 5.0 mmol/liter, but higher concentrations can be assayed by changing conditions slightly. Day-to-day reproducibility for the method was satisfactory (CV, 2.7 to 8.4%). Comparison of this method and two other methods for triglycerides, the automated Hantzsch condensation method and a commercial enzymic method (Calbiochem), gave correlation coefficients of 0.976 and 0.990, respectively. Results are unaffected by the presence of endogenous serum ADP, pyruvic acid, phosphatases, or ATPase.