Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bioanalytical considerations for compounds containing free sulfhydryl groups.

Nuggehally R Srinivas1, Rao N V S Mamidi

  • 1Department of Bioanalysis, Drug Metabolism and Pharmacokinetics, Discovery Research, Dr Reddy's Research Laboratories, Bollaram Road, Miyapur, Hyderabad-500 050, India. nrsrinivas@drreddys.com

Biomedical Chromatography : BMC
|July 29, 2003
PubMed
Summary

Developing robust bioanalytical methods for reactive thiol-containing compounds requires careful consideration of matrix stabilization and reagent selectivity. This review offers insights for validating methods for thiol drugs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacology and nonclinical development of teplizumab, a first-in-class immunotherapy for delaying the onset of type 1 diabetes.

The Journal of pharmacology and experimental therapeutics·2026
Same author

Development and Validation of an LC-MS/MS Method for the Quantitative Estimation of Bexicaserin in Human Cerebrospinal Fluid.

Biomedical chromatography : BMC·2026
Same author

An LC-MS/MS Method for the Quantitation of Metabolites M9, M12, and M20 of Bexicaserin in Human Cerebrospinal Fluid.

Biomedical chromatography : BMC·2026
Same author

Insights Influencing the Selection of Stable Isotopically Labeled Internal Standards (SIL-ISs) for LC-MS/MS Quantitative Assays.

Biomedical chromatography : BMC·2025
Same author

Safety, Tolerability, Pharmacokinetics, and Pharmacodynamics of Multiple Ascending Doses and Dose Titration of Bexicaserin in Healthy Participants in a Randomized, Double-Blind, Placebo-Controlled Study.

Clinical pharmacology in drug development·2025
Same author

Safety, Tolerability, Pharmacokinetics, Pharmacodynamics, and Food Effect of Bexicaserin in Healthy Participants: A First-in-Human Randomized, Double-Blind, Placebo-Controlled Single Ascending Dose Escalation Phase 1 Study.

Clinical pharmacology in drug development·2025

Area of Science:

  • Analytical Chemistry
  • Bioanalysis
  • Pharmaceutical Analysis

Background:

  • Thiol-containing compounds present significant bioanalytical challenges due to the high reactivity of their sulfhydryl groups.
  • Developing reliable methods necessitates addressing stabilization within biological matrices and ensuring selectivity.
  • A wide array of thiol reagents are available, offering potential solutions for these challenges.

Purpose of the Study:

  • To provide critical insights into the considerations for developing and validating bioanalytical methods for thiol-containing drugs.
  • To guide researchers in selecting appropriate strategies for handling reactive thiol compounds.
  • To enhance the robustness and reliability of thiol drug analysis.

Main Methods:

  • This is a review article, synthesizing existing knowledge and considerations.

Related Experiment Videos

  • It critically evaluates factors influencing method development for thiol compounds.
  • The focus is on strategic decision-making for method validation.

    • Main Results:

    • Key considerations for thiol bioanalysis include matrix effects, analyte stability, and reagent choice.
    • The selection of thiol-specific reagents is crucial for achieving selectivity and sensitivity.
    • A systematic approach to method development and validation is essential for reliable results.

    Conclusions:

    • Effective bioanalytical strategies for thiol drugs depend on a thorough understanding of sulfhydryl group reactivity.
    • Careful selection of reagents and validation parameters are vital for accurate quantification.
    • This review serves as a guide for overcoming common challenges in thiol bioanalysis.