[Cloning and characterization of mitochondrial NADH dehydrogenase gene of Cysticercus cellulosae]
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1Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017.
Objective:
To clone and characterize the NADH1 gene of Cysticercus cellulosae.
Methods:
A cDNA library was constructed from Cysticercus cellulosae and was immunoscreened by using rabbit anti-Cysticercus cellulosae polyclonal antibody. The gene structure and its possible function were analyzed by comparing with sequences available in the GenBank, after the insert of positive clone was subcloned and the nucleotide sequence of the insert was determined by dideoxynucleotide chain termination method.
Results And Conclusion:
A cDNA clone (named TS5) with a length of 1,082 bp was isolated. The 5' terminal of cloned gene contained one open reading frame of 1-578 bp encoding 192 amino acid residues of mitochondrial NADH dehydrogenase subunit 1 and the 3' terminal contained three kinds of tRNA genes.
