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Growing and working with peripheral neurons.

Yan He1, Peter W Baas

  • 1Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA.

Methods in Cell Biology
|July 30, 2003
PubMed
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This chapter details methods for culturing vertebrate peripheral neurons, specifically rat sympathetic and chick sensory neurons. It covers essential techniques for successful neuron cell biology research.

Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Vertebrate peripheral neuron cultures are vital for studying neuronal cell biology.
  • These cultures allow for experimental manipulations like microinjection.
  • Various culture conditions can be optimized for specific research needs.

Purpose of the Study:

  • To provide comprehensive methods for culturing rat sympathetic and chick sensory neurons.
  • To detail essential techniques for maintaining healthy and viable neuronal cultures.
  • To guide researchers in optimizing culture conditions for experimental success.

Main Methods:

  • Dissection of superior cervical ganglia (rat) and lumbosacral dorsal root ganglia (chick).
  • Preparation of culture dishes and appropriate substrates.
  • Optimization of media composition, including essential growth factors.

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  • Strategies for minimizing nonneuronal cell contamination.
  • Protocols for long-term maintenance of neuronal cultures.

    • Main Results:

    • Established protocols for successful primary cultures of rat sympathetic and chick sensory neurons.
    • Demonstrated methods for controlling culture environment and cell purity.
    • Provided a foundation for further experimental investigations using these neuronal models.

    Conclusions:

    • Primary cultures of vertebrate peripheral neurons are a powerful tool for neuroscience research.
    • Detailed methodologies presented enable reliable and reproducible neuronal culture experiments.
    • These techniques support diverse research applications in neuronal cell biology and function.