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H2-O influence on antigen presentation in H2-E-expressing mice.
Christopher Alfonso1, G Stuart Williams, Lars Karlsson
1Johnson & Johnson Pharmaceutical Research & Development, 3210 Merryfield Row, San Diego, CA 92121, USA.
European Journal of Immunology
|July 30, 2003
Summary
MHC class II molecule presentation is modulated by HLA-DO (H2-O) in B cells. H2-Ed/b expression influences H2-Ab epitopes, with H2-O further altering this peptide repertoire.
Area of Science:
- Immunology
- Molecular Biology
Background:
- MHC class II molecules present peptides to CD4+ T cells.
- HLA-DM (DM) facilitates peptide loading in endosomes.
- In B cells, DM associates with HLA-DO (DO).
Purpose of the Study:
- Investigate H2-O's influence on H2-Ed/b molecule epitope presentation.
- Examine H2-O's effect on H2-Ab epitope presentation in H2-Ead-transgenic mice.
- Determine the impact of H2-Ed/b and H2-O co-expression on B cell antigen presentation.
Main Methods:
- Utilized H2-Ead-transgenic mouse models.
- Analyzed antigen presentation following membrane immunoglobulin-mediated uptake.
- Compared peptide repertoires in H2-O-deficient mice.
Main Results:
- H2-O influences H2-Ed/b molecule epitope presentation.
- Co-expression of H2-Ab and H2-Ed/b in B cells alters H2-Ab-restricted epitope presentation.
- Absence of H2-O leads to further changes in the peptide repertoire.
Conclusions:
- H2-Ed/b expression modulates the epitopes presented by H2-Ab molecules.
- H2-O significantly alters the peptide repertoire presented by MHC class II molecules in B cells.