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Solid phase synthesis of phosphorothioate oligonucleotides utilizing diethyldithiocarbonate disulfide (DDD) as an
Zacharia S Cheruvallath1, R Krishna Kumar, Claus Rentel
1Isis Pharmaceuticals, Carlsbad, California 92008, USA.
Nucleosides, Nucleotides & Nucleic Acids
|July 30, 2003
Abstract:
Diethyldithiodicarbonate (DDD), a cheap and easily prepared compound, is found to be a rapid and efficient sulfurizing reagent in solid phase synthesis of phosphorothioate oligodeoxyribonucleotides via the phosphoramidite approach. Product yield and quality based on IP-LC-MS compares well with high quality oligonucleotides synthesized using phenylacetyl disulfide (PADS) which is being used for manufacture of our antisense drugs.