Differential expression of sphingolipids in P-glycoprotein or multidrug resistance-related protein 1 expressing human neuroblastoma cell lines
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Updated: Sep 20, 2026
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
Published on: June 8, 2022
Anne-Jan Dijkhuis1, Jenny Douwes, Willem Kamps
1Department of Membrane Cell Biology, Groningen University Institute for Drug Exploration (GUIDE), University of Groningen, A. Deusinglaan 1, Bldg. 3215, 10th Fl., 9713 AV, Groningen, The Netherlands. a.dijkhuis@med.rug.nl
Abstract:
The sphingolipid composition and multidrug resistance status of three human neuroblastoma cell lines were established. SK-N-FI cells displayed high expression and functional (efflux) activity of P-glycoprotein, while multidrug resistance-related protein 1 was relatively abundant and most active in SK-N-AS cells. These two cell lines exhibited higher sphingolipid levels, compared to SK-N-DZ, which had the lowest activity of either ATP-binding cassette transporter protein. SK-N-DZ cells also differed in ganglioside composition with predominant expression of b-series gangliosides. In conclusion, these three neuroblastoma cell lines offer a good model system to study sphingolipid metabolism in relation to ATP-binding cassette transporter protein function.