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The shape parameter of liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes
Y Sun1, X Li, N Düzgüneş
1Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China.
Biophysical Journal
|July 30, 2003
Abstract:
A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/-) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.