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Specific IgG antibody responses in experimental cat metagonimiasis
Seung Yull Cho1, Suk Il Kim, Shin Yong Kang
1Department of Parasitology, College fo Medicine, Chung-Ang University, Seoul, Korea.
Kisaengch'Unghak Chapchi. the Korean Journal of Parasitology
|December 1, 1987
Summary
Serologic diagnosis of metagonimiasis is feasible using ELISA. Adult worm antigen is more effective than metacercarial antigen for detecting specific IgG antibodies in infected cats.
Area of Science:
- Parasitology
- Immunology
- Veterinary Medicine
Background:
- Metagonimiasis is a food-borne parasitic disease caused by Metagonimus yokogawai.
- Accurate diagnostic methods are crucial for controlling metagonimiasis.
- Serological assays offer a potential route for diagnosing parasitic infections.
Purpose of the Study:
- To evaluate the feasibility of serologic diagnosis for metagonimiasis.
- To compare the efficacy of different antigens (metacercarial vs. adult worm) in detecting specific antibodies.
Main Methods:
- Preparation of saline extracts from metacercariae and adult worms of Metagonimus yokogawai.
- Collection of sera from experimentally infected cats at various time points post-infection.
- Quantification of specific IgG antibody levels using enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Specific IgG antibody levels rose within 7 days post-infection, peaking at 2-4 weeks.
- Antibody levels plateaued after the peak response.
- Adult worm antigen demonstrated superior performance in detecting specific IgG antibodies compared to metacercarial antigen.
- Cats with heavy worm burdens showed a minimal antibody rise.
Conclusions:
- Serologic diagnosis of metagonimiasis is feasible.
- Adult worm antigen is a more sensitive biomarker for detecting metagonimiasis-specific IgG antibodies.
- ELISA using adult worm antigen can be a valuable tool for diagnosing metagonimiasis.