Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualizing membrane trafficking using total internal reflection fluorescence microscopy.

V Beaumont1

  • 1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK. vahri@mrc-lmb.cam.ac.uk

Biochemical Society Transactions
|July 31, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco).

Molecular ecology resources·2011
Same author

Roles for mitochondrial and reverse mode Na+/Ca2+ exchange and the plasmalemma Ca2+ ATPase in post-tetanic potentiation at crayfish neuromuscular junctions.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
Same author

Phosphorylation and local presynaptic protein synthesis in calcium- and calcineurin-dependent induction of crayfish long-term facilitation.

Neuron·2001
Same author

Detection of tyrosine phosphorylated proteins in Trichinella spiralis L1 larvae.

Parasite (Paris, France)·2001
Same author

Enhancement of synaptic transmission by cyclic AMP modulation of presynaptic Ih channels.

Nature neuroscience·2000
Same author

Somatostatin receptor desensitization in NG108-15 cells. A consequence of receptor sequestration.

The Journal of biological chemistry·1998
Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026
Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026
Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026
Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026
Same journal

Implications of Rho GTPase signaling in cancer immunotherapy.

Biochemical Society transactions·2026
See all related articles

Total internal reflection fluorescence microscopy (TIR-FM) offers high-resolution insights into live cell plasma membrane events. This technique is increasingly vital for studying exocytosis and endocytosis regulation in excitable cells.

Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Advances in fluorescent probes and protein labeling have fueled the popularity of fluorescence microscopy.
  • Total internal reflection fluorescence microscopy (TIR-FM) provides high optical resolution for studying live cell plasma membrane events.

Purpose of the Study:

  • To review the recent literature on TIR-FM applications in membrane trafficking.
  • To emphasize the use of TIR-FM in studying exocytosis and endocytosis regulation in excitable cells.

Main Methods:

  • Review of scientific literature focusing on TIR-FM.
  • Application of TIR-FM to observe cellular processes at the plasma membrane.

Main Results:

  • TIR-FM has seen significant expansion in its application to membrane trafficking in both non-excitable and excitable cells.

Related Experiment Videos

  • The technique allows detailed study of exocytosis and endocytosis regulation.

    • Conclusions:

    • TIR-FM is a powerful tool for investigating cellular processes at the molecular level.
    • The future of TIR-FM in cell biology research is promising for uncovering new insights.