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Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
Bone marrow stromal cells attenuate mitoxantrone cytotoxicity against HL-60 leukemic cells
A Takahashi1, K Yamamoto, M Okuma
1Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Abstract:
We evaluated the effect of cultured human bone marrow (BM) stromal cells on the cytotoxicity of mitoxantrone (MIT) against the HL-60 leukemic cell line. BM-derived fibroblastoid cells (BMFC) suppressed colony formation of HL-60 cells in the absence of MIT. BMFC increased the survival of HL-60 colony-forming cells in the presence of 50 ng/ml MIT. A significant decrease in the drug concentration was not detected on BMFC. Our results suggest that BM stromal cells can modulate the antileukemic effect of an anticancer drug.
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