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Pulp cell responses during hypoxia and reoxygenation in vitro
Kei Amemiya1, Yuzuru Kaneko, Takashi Muramatsu
1Department of Dental Anesthesiology, Tokyo Dental College, Chiba-city, Japan. kamemiya@tdc.ac.jp
European Journal of Oral Sciences
|July 31, 2003
Summary
Pulp cells show increased growth and alkaline phosphatase activity under hypoxia. Reoxygenation normalizes these but increases heat shock protein 70, indicating dynamic responses to oxygen changes.
Area of Science:
- Biomedical Sciences
- Cell Biology
- Dental Research
Background:
- Pulp cells are crucial for dental health.
- Understanding cellular responses to oxygen deprivation and restoration is vital for regenerative endodontics.
Purpose of the Study:
- To investigate the dynamic responses of pulp cells to hypoxia and reoxygenation.
- To analyze changes in cell viability, proliferation, alkaline phosphatase activity, and specific protein expressions.
Main Methods:
- Cultured beagle dog pulp tissues under normoxic, hypoxic, and reoxygenation conditions.
- Assessed cell viability, MTT reduction, proliferation, and alkaline phosphatase (ALPase) activity.
- Analyzed heat shock protein 70 (HSP70), vascular endothelial growth factor (VEGF), and hypoxia-inducible factor-1alpha (HIF-1alpha) expression.
Main Results:
- Hypoxia significantly increased pulp cell proliferation and ALPase activity.
- Reoxygenation led to decreased proliferation and ALPase activity, but increased HSP70 expression.
- HIF-1alpha and VEGF expression increased under hypoxic conditions.
Conclusions:
- Pulp cells exhibit dynamic adaptive responses to both hypoxic and reoxygenation stress.
- These findings provide insights into cellular mechanisms during oxygen fluctuations in dental pulp.