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Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
06:40

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

Antimicrobial resistance in Helicobacter pylori isolates from children

S Rerksuppaphol1, W Hardikar, P D Midolo

  • 1Department of Paediatric Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Austin Hospital, Melbourne, Victoria, Australia.

Journal of Paediatrics and Child Health
|July 31, 2003
PubMed

Insights

Helicobacter pylori resistance to metronidazole is high in children, while clarithromycin resistance is moderate. Amoxycillin and tetracycline resistance were not observed, suggesting treatment modifications may be needed.

Area of Science:

  • Pediatric infectious diseases
  • Microbiology
  • Antimicrobial resistance

Background:

  • Helicobacter pylori infection is common in children.
  • Treatment of H. pylori relies on effective antimicrobial agents.
  • Understanding antimicrobial resistance patterns is crucial for effective treatment.

Purpose of the Study:

  • To determine the antimicrobial resistance rates of H. pylori in pediatric patients.
  • To assess resistance to metronidazole, clarithromycin, amoxycillin, and tetracycline.

Main Methods:

  • Prospective collection of H. pylori isolates from children undergoing gastroscopy (July 1997-January 2000).
  • Susceptibility testing using E-test for four antimicrobial agents.
  • Retrospective collection of demographic, clinical, and treatment data.

Main Results:

  • High resistance rate to metronidazole (43.5%) and moderate resistance to clarithromycin (8.7%).
  • No resistance observed for amoxycillin or tetracycline.
  • No significant differences in patient characteristics or treatment outcomes between susceptible and resistant groups.

Conclusions:

  • Pediatric H. pylori resistance patterns to metronidazole and clarithromycin mirror adult data.
  • High resistance rates necessitate consideration of antimicrobial susceptibility testing for H. pylori management in children.
  • Treatment guidelines may require updates to ensure eradication efficacy.
Abstract

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