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Updated: Sep 20, 2026

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
Published on: October 24, 2016
[Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray]
Da-nian Tang1, Yong-guo Li, Jun-min Wei
1Department of Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.
Methods:
The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).
Results:
Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.
Conclusions:
cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.
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DNA Microarrays
Cell Specific Gene Expression

