A new mitochondrial fluorescent zinc sensor
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Published on: June 2, 2023
Stefano L Sensi1, Dien Ton-That, John H Weiss
1Department of Neurology, University of California, Irvine, CA 92697-4292, USA.
Abstract:
A novel cationic fluorescent zinc (Zn2+) indicator (RhodZin-3) with nanomolar affinity for Zn2+ has been synthesized. RhodZin-3 exhibits large pH-independent fluorescence increases in the orange region of the visible wavelength spectrum with increasing zinc concentrations, and no sensitivity to physiologically relevant Ca2+ concentrations. Experiments in neuronal cell cultures show that RhodZin-3 effectively localizes into mitochondria and detects changes of intramitochondrial free Zn2+ ([Zn2+]m).
