Related Experiment Video
Updated: Sep 18, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Aberrant methylation in promoter-associated CpG islands of multiple genes in therapy-related leukemia
Eisuke Uehara1, Seisho Takeuchi, Taizo Tasaka
1First Department of Internal Medicine, Kagawa Medical University, Miki-cho, Kagawa 761-0793, Japan.
Abstract:
Methylation profile was analyzed in eleven cases of therapy-related leukemia (t-leukemia) for p14, p15, p16, Rb, hMLH1, hMSH2, MGMT, APC, RAR beta, DAPK, RIZ1, FHIT, and SOCS-1 genes by using methylation specific polymerase chain reaction (MSP) analysis. Six (55%) of eleven cases showed methylation of at least one gene. The average time to the development of t-leukemia after the treatment of the primary tumor was significantly shorter in patients with methylation than those without methylation (49.3 months vs. 133.2 months, P=0.044). These results suggest that hypermethylation might be involved in the development of t-leukemia.
More Related Videos
Related Concept Videos
Epigenetic Regulation
Abnormal Proliferation
Targeted Cancer Therapies
There are several types of targeted therapies against specific...
Combination Therapies and Personalized Medicine
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
Epigenetic Regulation
X-chromosome...
Induced Pluripotent Stem Cells
Somatic cells are...

