Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum

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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Prakash Bhosale1, Igor V Ermakov, Maia R Ermakova

  • 1Department of Ophthalmology and Visual Sciences, 50 North Medical Drive, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.

Biotechnology Letters
|August 2, 2003
PubMed

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Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...