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Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum
Prakash Bhosale1, Igor V Ermakov, Maia R Ermakova
1Department of Ophthalmology and Visual Sciences, 50 North Medical Drive, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Biotechnology Letters
|August 2, 2003
Abstract:
Resonant Raman scattering was used as a novel, rapid, non-destructive optical technique to measure zeaxanthin levels in Flavobacterium multivorum ATCC 55238. Culture broth, after bacterial growth for 40 h, exhibited characteristic resonance Raman vibrational modes at 1159 cm(-1) (C-C stretch) and 1525 cm(-1) (C=C stretch) upon excitation at 488 nm. A striking correlation was observed between the carotenoid level as estimated by HPLC and by resonance Raman spectroscopy.