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[Detection of platelet Ca2+(i), CD62P, CD63 and plasma CD62P in cirrhosis patients]
Zhong Tang1, Jing-guo Zhuo, Wen-fang Huang
1Department of Clinical Laboratory, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China.
Insights
Cirrhosis patients show significantly elevated platelet activation markers, including intracellular calcium (Ca2+(i)), CD62P, CD63, and serum CD62P (SCD62P). These markers are also higher in cirrhosis patients with gastrointestinal bleeding, indicating increased platelet activity.
Area of Science:
- Biochemistry
- Hematology
- Pathophysiology
Background:
- Cirrhosis is a severe liver condition associated with various complications.
- Platelet activation plays a role in thrombotic and inflammatory processes in liver disease.
- Understanding platelet behavior in cirrhosis is crucial for assessing disease severity and predicting complications.
Purpose of the Study:
- To investigate the relationship between platelet intracellular calcium (Ca2+(i)), CD62P, CD63 expression, and serum CD62P (SCD62P) levels in patients with cirrhosis.
- To compare these markers between cirrhosis patients and healthy controls.
- To evaluate the association of these markers with upper gastrointestinal hemorrhage in cirrhosis.
Main Methods:
- Platelet CD62P and CD63 expression were quantified using flow cytometry.
- Serum CD62P (SCD62P) levels were measured via ELISA.
- Intracellular calcium (Ca2+(i)) in platelets was determined using fluorophotometry.
Main Results:
- Cirrhosis patients exhibited significantly higher levels of platelet Ca2+(i), CD62P, CD63, and SCD62P compared to healthy controls (P<0.05).
- Patients with upper gastrointestinal hemorrhage showed markedly elevated levels of all measured platelet markers compared to those without hemorrhage (P<0.05).
- No significant difference in platelet count was observed between the hemorrhage and non-hemorrhage groups.
Conclusions:
- Platelets are significantly activated in patients with cirrhosis.
- The detection of platelet CD62P, CD63, and SCD62P is valuable for assessing the degree of cirrhosis.
- These markers may serve as indicators of increased thrombotic risk and potential complications like gastrointestinal bleeding in cirrhosis.
Objectives:
To study on the relationship between platelet Ca2+(i), CD62P, CD63, serum CD62P (SCD62P) and cirrhosis patients.
Methods:
Platelet CD62P, CD63 were determined with flow cytometry, SCD63P with ELISA, and Ca2+(i) in platelet was determined with fluorophotometry.
Results:
Platelet Ca2+(i), CD62P, CD63, and SCD62P levels in cirrhosis patients were (103.1+/-22.2)nmol/L, (47.6+/-20.0)%, (47.1+/-24.6)%, and (67.6+/-37.6)microg/L, and in controls were (57.6+/-13.1)nmol/L, (3.1+/-0.7)%, (2.5+/-0.7)%, and (24.0+/-6.5)microg/L, respectively. The levels in the former were higher than those in the latter (t > or = 6.148, P<0.05). The above levels in upper gastrointestinal haemorrhage group were much higher than those in the non-haemorrhage group (120.3nmol/L+/-18.8nmol/L vs 91.1nmol/L+/-14.3nmol/L, 64.9%+/-14.7% vs 34.6%+/- 11.9%, 70.9%+/-14.5% vs 30.2%+/-14.4%, and 103.6microg/L+/-14.9microg/L vs 40.8microg/L+/-24.0microg/L, respectively, t > or = 5.380, P<0.05). But the numbers of platelet between the two groups were no obvious difference.
Conclusions:
Platelet in the cirrhosis patients is greatly active, and the detection of platelet CD62P, CD63, SCD62P has a certain value in judging the degree of cirrhosis.
Related Concept Videos
01:24Cirrhosis II: Pathophysiology
01:23Cirrhosis I: Introduction