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Surface trapping and AFM detection of DNA topological intermediates generated from an oxidative chemical nuclease

R Mukhopadhyay1, S G Srivatsan, S Verma

  • 1Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh 208016, India. rupam@iitk.ac.in

Biochemical and Biophysical Research Communications
|August 2, 2003
PubMed

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Nucleotide Excision Repair

Exposure to mutagens can damage DNA and result in bulky lesions that distort the double-helix structure or impede proper transcription. Damaged DNA can be detected and repaired in a process called nucleotide excision repair (NER). NER employs a set of specialized proteins that first scan DNA to detect a damaged region. Next, NER proteins separate the strands and excise the damaged area. Finally, they coordinate the replacement with new, matching nucleotides.DNA distortion and damageCells are...