Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Galpha12 regulates epithelial cell junctions through Src tyrosine kinases.

Tobias N Meyer1, Jennifer Hunt, Catherine Schwesinger

  • 1Renal Division, Brigham and Women's Hospital and Harvard Medical School, Harvard Institutes of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

American Journal of Physiology. Cell Physiology
|August 2, 2003
PubMed
Summary

G protein Galpha12 disrupts epithelial cell junctions via Src tyrosine kinase pathways. Inhibiting Src kinase prevents Galpha12-induced junctional protein disruption and loss of cell polarity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of Structured Exercise Program with Resistance Training and Verticalization in Adults on Extracorporeal Membrane Oxygenation: A Prospective Pilot Study.

Critical care medicine·2026
Same author

Autoantibody-triggered podocyte membrane budding drives autoimmune kidney disease.

Cell·2025
Same author

The Utility of Collaborative Teamwork for Nursing Students in the Acute Care Setting.

Nursing education perspectives·2025
Same author

Correction: Cardiac physiology and pathophysiology in pregnancy.

Canadian journal of physiology and pharmacology·2024
Same author

Resection of Multiple Left Ventricular Fibroelastomas Identified in a Pregnant Patient With Mechanical Left-Side Heart Valves.

The Canadian journal of cardiology·2024
Same author

Incidence, Recognition, and Follow-up of Laboratory Evidence of Acute Kidney Injury in Primary Care Practices: Analysis of 93,259 Creatinine Results.

The American journal of medicine·2024

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Epithelial Biology

Background:

  • Epithelial cell junctions are regulated by signaling pathways, including heterotrimeric G proteins.
  • Previous work showed Galpha12 binds to ZO-1 and increases paracellular permeability in MDCK cells.

Purpose of the Study:

  • To investigate the effects of Galpha12 expression on tight and adherens junction proteins.
  • To examine the downstream signaling pathways regulated by Galpha12 in epithelial cells.

Main Methods:

  • Confocal microscopy to visualize junctional proteins and actin stress fibers in MDCK cells.
  • Treatment with tyrosine kinase inhibitors (genistein) and Src-specific inhibitor (PP-2).
  • Measurement of paracellular permeability using transepithelial resistance and mannitol flux.

Related Experiment Videos

  • Assessment of Src activity via autophosphorylation and beta-catenin tyrosine phosphorylation.

    • Main Results:

    • Constitutively active Galpha12 (QLalpha12) expression disrupted tight and adherens junctions, increased actin stress fibers, and altered ZO-1/Na-K-ATPase distribution, indicating loss of polarity.
    • Genistein and PP-2 treatment abrogated the QLalpha12 phenotype, preserving junctional protein localization and preventing increased paracellular permeability.
    • QLalpha12 expression increased Src activity and beta-catenin tyrosine phosphorylation without affecting Rho activity.

    Conclusions:

    • Galpha12 regulates epithelial cell junctions in Madin-Darby canine kidney (MDCK) cells.
    • The Src tyrosine kinase pathway is a key mediator of Galpha12's effects on cell junctions.
    • Galpha12-induced disruption of epithelial polarity and permeability is dependent on Src kinase activity.