Unstructured model for L-lysine fermentation under controlled dissolved oxygen
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Updated: Sep 20, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Semsi Ensari1, Joon Ha Kim, Henry C Lim
1Biotechnology Development, Schering-Plough Research Institute, 1011 Morris Avenue, U-14-2-20, Union, New Jersey 07083, USA. semsi.ensari@spcorp.com
Abstract:
An unstructured model was developed for batch cultivation of Corynebacterium lactofermentum (ATCC 21799) under controlled dissolved oxygen. The model is capable of predicting batch experiments performed at various initial substrate concentrations. By extending the batch culture model to a fed-batch model and using a heuristic approach to optimize the fed-batch cultivation, it is shown that fed-batch cultivation is superior to batch operation due to increased productivity at high substrate concentrations.
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