Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The proteasomal system and HNE-modified proteins.

Tilman Grune1, Kelvin J A Davies

  • 1Neuroscience Research Center, Medical Faculty (Charité), Humboldt University, Schumannstrasse 20/21, 10117 Berlin, Germany. tilman.grune@charite.de

Molecular Aspects of Medicine
|August 2, 2003
PubMed
Summary

Oxidative stress generates reactive aldehydes like 4-hydroxynonenal (HNE) that modify proteins. While the proteasome degrades some modified proteins, extensive HNE modification can form aggregates, inhibiting proteasome function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Dose Intravenous Vitamin C and Mortality and Organ Dysfunction in Severe Burn Injury: The VICTORY Randomized Clinical Trial.

JAMA·2026
Same author

Biologically Younger Individuals, as Identified by MARK-AGE Biological Age Scores, Display a Distinct Favourable Blood Chemistry Profile Regardless of Age.

Aging cell·2026
Same author

Dysregulated Klotho and FGF23 signalling aggravates vascular remodelling in age-related pulmonary hypertension.

Cardiovascular research·2026
Same author

Corrigendum to "Prevention of colitis-induced liver oxidative stress and inflammation in a transgenic mouse model with increased omega-3 polyunsaturated fatty acids" [Redox Biol. 64 (2023) 102803].

Redox biology·2026
Same author

Resistance-based training improves mitochondrial capacity and redox balance in aging adults, independent of polyphenol supplementation.

Redox biology·2026
Same author

Comparative analysis of metabolic and functional cardiac alterations in diet- and genetically induced mouse models of cardiac dysfunction.

The FEBS journal·2025

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Oxidative Stress Research

Background:

  • Metabolic processes and environmental factors generate reactive oxygen species, leading to cellular component oxidation.
  • Lipid peroxidation produces reactive aldehydes, such as 4-hydroxynonenal (HNE), which modify proteins.
  • Oxidized and modified proteins require degradation as part of cellular defense mechanisms.

Purpose of the Study:

  • To review the interactions between lipid peroxidation products, proteins, and the proteasomal system.
  • To elucidate the role of the proteasome in degrading oxidatively modified proteins.
  • To understand how HNE modification impacts protein degradation and proteasome function.

Main Methods:

  • Literature review of studies on oxidative stress, lipid peroxidation, protein modification, and proteasomal degradation.

Related Experiment Videos

  • Analysis of research detailing the effects of 4-hydroxynonenal (HNE) on protein structure and function.
  • Examination of the mechanisms by which the proteasome recognizes and degrades modified proteins.

    • Main Results:

    • Reactive aldehydes from lipid peroxidation, like HNE, covalently modify intracellular proteins.
    • The proteasomal system is the primary pathway for degrading oxidized cytosolic and nuclear proteins.
    • Mild HNE modification facilitates proteasomal degradation, whereas extensive modification leads to protein aggregates that inhibit the proteasome.

    Conclusions:

    • The proteasome's ability to degrade HNE-modified proteins is concentration-dependent.
    • Extensive protein modification by HNE can overwhelm cellular degradation capacity, leading to proteasome inhibition and potential cellular dysfunction.
    • Understanding these interactions is crucial for comprehending cellular responses to oxidative stress.