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Related Experiment Videos

Histone phosphorylation: how to proceed.

Romain Loury1, Paolo Sassone-Corsi

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS, INSERM, Université Louis Pasteur, B.P. 10142, Illkirch, Strasbourg 67404, France.

Methods (San Diego, Calif.)
|August 2, 2003
PubMed
Summary
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Histone phosphorylation links chromatin remodeling to cell signaling. New methods like in-gel and in vitro kinase assays help identify kinases involved in these crucial cellular processes.

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Epigenetics

Background:

  • Posttranslational modifications of histone tails are critical for cellular processes.
  • Histone phosphorylation directly links chromatin remodeling with intracellular signaling pathways.
  • Specific serine residues on histone tails are targets for kinases, influencing gene expression and chromatin condensation.

Purpose of the Study:

  • To describe experimental methodologies for studying histone phosphorylation.
  • To identify kinases involved in specific signaling pathways affecting chromatin.
  • To understand the role of histone phosphorylation in the 'histone code' and physiological responses.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) for identifying gene targets.

Related Experiment Videos

  • In-gel kinase assays for initial kinase identification.
  • In vitro kinase assays for validating candidate kinases.

    • Main Results:

    • Established methodologies for investigating histone phosphorylation.
    • Demonstrated the utility of ChIP, in-gel kinase assays, and in vitro kinase assays.
    • Provided a framework for identifying kinases regulating chromatin remodeling.

    Conclusions:

    • Histone phosphorylation is a key regulatory mechanism.
    • The described methods are essential for deciphering kinase-mediated signaling in chromatin remodeling.
    • Understanding these pathways is critical for comprehending cellular processes.