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Related Experiment Videos

Extracellular superoxide dismutase polymorphism in mice.

Anson Pierce1, Jason Whitlark, Ladislav Dory

  • 1Department of Molecular Biology & Immunology, University of North Texas Health Science Center at Fort Worth, USA.

Arteriosclerosis, Thrombosis, and Vascular Biology
|August 2, 2003
PubMed
Summary

Researchers identified a new extracellular superoxide dismutase (ecSOD) allele in mice. This allele significantly impacts ecSOD activity, highlighting the importance of genetic background in research.

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Area of Science:

  • Genetics
  • Biochemistry
  • Mouse Models

Background:

  • Extracellular superoxide dismutase (ecSOD) plays a crucial role in antioxidant defense.
  • Understanding genetic variations in ecSOD is essential for interpreting research findings, particularly in mouse models.

Purpose of the Study:

  • To identify and characterize a novel murine extracellular superoxide dismutase (ecSOD) allele.
  • To investigate the distribution of this ecSOD allele across different mouse strains.
  • To determine the impact of the identified ecSOD allele on the ecSOD phenotype.

Main Methods:

  • Polymerase chain reaction (PCR) analysis of genomic and cDNA.
  • DNA sequencing and genotyping.
  • Measurement of free and heparin-releasable ecSOD activities.

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  • Immunoblotting to assess plasma enzyme mass.

    • Main Results:

    • Two distinct ecSOD transcripts and alleles were identified: a short variant (10-bp deletion in 3'UTR and a single nucleotide substitution) and a long form.
    • The 129P3/J strain carries the short ecSOD allele, while other strains carry the long form.
    • Mice with the short ecSOD allele exhibit over 3-fold higher ecSOD activity and enzyme mass compared to mice with the long allele.
    • A clear allele dose-dependent effect on ecSOD activity was observed in F2 hybrid mice.

    Conclusions:

    • Allele-specific effects on the ecSOD phenotype are demonstrated, independent of other strain-specific factors.
    • The findings underscore the necessity of backcrossing genetically modified mice to control for genetic background variations.
    • Accurate characterization of ecSOD alleles is critical for reproducible research in cardiovascular and metabolic disease models.