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Expression profiling of tumor necrosis factor alpha-induced apoptosis-associated genes in human solid tumor cell

Yoshiaki Yanai1, Mark J Micallef, Shigeto Yamamoto

  • 1Hayashibara Biochemical Laboratories Inc., Fujisaki Institute, Fujisaki 675-1, Okayama 702-8006, Japan.

Anticancer Research
|August 5, 2003
PubMed
Abstract

Insights

Tumor necrosis factor-alpha (TNF-alpha) can induce apoptosis in cancer cells. Akt mRNA expression levels may predict which cancer cell lines respond to TNF-alpha, aiding in targeted therapy selection.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Tumor necrosis factor-alpha (TNF-alpha) induces apoptosis in human cancer cell lines.
  • Identifying TNF-alpha responder cell lines is crucial for cancer therapy.
  • TNF-alpha response is linked to specific genetic expression profiles.

Purpose of the Study:

  • To elucidate relationships between TNF-alpha response and gene expression profiles.
  • To identify potential genetic markers for predicting TNF-alpha responsiveness.
  • To explore the role of gene expression in TNF-alpha-induced apoptosis.

Main Methods:

  • Quantitative real-time polymerase chain reaction (PCR) was employed.
  • Comprehensive molecular profiling of 91 human cancer cell lines was performed.
  • Gene expression downstream of TNF-alpha receptor genes was analyzed.

Main Results:

  • Above-average Akt mRNA expression correlated significantly with TNF-alpha-induced apoptosis susceptibility.
  • Multidrug resistance protein 5 (MRP5) mRNA expression may indicate TNF-alpha responsiveness.
  • Tumor necrosis factor receptor type 1 (TNFR1) mRNA expression is a potential marker for TNF-alpha responsiveness.

Conclusions:

  • Genetic markers can predict favorable therapeutic outcomes in cancer patients.
  • Identifying patients who benefit from cytokine therapy is possible.
  • This study provides a preliminary basis for screening predictive genetic markers.

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