Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ets-2 interacts with co-repressor BS69 to repress target gene expression.

Guo Wei1, Alicia Erbe Schaffner, Kimberly M Baker

  • 1Department of Molecular Genetics, Comprehensive Cancer Center, Ohio State University, 484 W. 12th Ave., Columbus, OH 43210, USA.

Anticancer Research
|August 5, 2003
PubMed
Summary

Phosphorylation of Ets-2 (a protein regulating cell growth) influences its interaction with co-repressor BS69, potentially switching Ets-2

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gradient-Guided Graph Contrastive Learning for Mass Spectrometry-Based Proteomics Clustering.

Journal of chemical information and modeling·2026
Same author

Cardiorenal Syndrome and Depressive Symptoms: Exploring the Mood-Kidney Link With Heart Failure Risk in a Post-hoc Analysis of SPRINT.

Kidney medicine·2026
Same author

A comprehensive review on chemical structure, quality control, pharmacological effects, and structure-activity relationship of saponins from Zhuzishen ().

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan·2026
Same author

Clinical Characteristics and Long-Term Outcomes of Patients With Fibrosing Mediastinitis: A Multicenter Retrospective Cohort Study.

JACC. Asia·2026
Same author

Association Between a History of Contact Sport Participation and Higher Lifetime Mild Traumatic Brain Injury Burden Among Military Servicemembers and Veterans: A Long-term Impact of Military-relevant Brain Injury Consortium-Chronic Effects of Neurotrauma Consortium Prospective Longitudinal Study (LIMBIC-PLS).

Orthopaedic journal of sports medicine·2026
Same author

Clinical Impact and Lesion Characteristics of Suboptimal Optical Coherence Tomography Stent Deployment in Patients With Acute Myocardial Infarction.

Journal of the American Heart Association·2026

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Transcription Factors

Background:

  • The ETS-family of transcription factors (over 30 members) regulates critical cellular processes like growth, differentiation, and survival.
  • Specificity within the ETS-family is poorly understood, with signaling pathway modulation being a key proposed mechanism.
  • Ets-2, an ETS-family member, is known to be modulated by phosphorylation, particularly in its pointed domain.

Purpose of the Study:

  • To investigate how phosphorylation of the Ets-2 pointed domain affects its interactions with other proteins in the nucleus.
  • To elucidate the role of identified interacting proteins in regulating Ets-2 target genes.

Main Methods:

  • Yeast two-hybrid screens to identify Ets-2 interacting proteins.
  • Biochemical assays (in vitro) to characterize protein-protein interactions.

Related Experiment Videos

  • Transfection assays to assess gene regulation by Ets-2 and its partners.

    • Main Results:

    • BS69, a known co-repressor, was identified as an Ets-2 interacting partner.
    • Phosphorylation of the Ets-2 pointed domain reduced its interaction with BS69 in vitro.
    • Co-expression of Ets-2 and BS69 led to repression of specific Ets-2 target genes in transfection assays.

    Conclusions:

    • Ets-2 can function as a repressor, requiring BS69 as a co-repressor.
    • Phosphorylation of Ets-2 may alter its function from repressor to activator by disrupting the Ets-2/BS69 complex formation.
    • This study provides insight into the regulatory mechanisms governing ETS-family transcription factor specificity.