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Updated: Sep 20, 2026

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
Published on: August 11, 2014
[Intracellular survival of Salmonella strains in Caco-2 cells]
Ute Dinjus1, Ingrid Hänel, Günter Steinbach
1Bundesforschungsanstalt für Viruskrankheiten der Tiere, Standort Jena. u.dinjus@jena.bfav.de
Abstract:
In the in vitro model using Caco-2 cells at different stages of differentiation the invasion and intracellular survival of virulent (predominant infection strains) and less virulent (predominant attenuated mutant strains) Salmonella strains were studied. The statistical evaluation of experimental data has shown that the logarithmized colony forming unit after 18 hours of incubation in differentiated cells (14 days old) is a suitable parameter for the determination of intracellular survival. Using this parameter a relationship between intracellular survival and Salmonella virulence (LD50 mouse) was demonstrated and quantified. The model presented could be suitable for the replacement of animal experiments after further investigations.

