Related Experiment Video
Updated: Sep 16, 2026
Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
Published on: November 18, 2019
[Study on the mitochondrion DNA mutation in tumor tissues of gynecologic oncology patients]
Hong-xia Li1, Sheng Zhong, Chun-hai Li
1Department of Obstetrics and Gynecology, BeiJing Railway General Hospital, Beijing 100038, China.
Objective:
To investigate the mitochondrion DNA (mtDNA) mutation in tumor tissues of gynecologic oncology patients and their relationship to tumorigenesis and tumor development.
Methods:
The samples of tumor tissue and their proximal normal tissue of 32 gynecological malignant tumor patients as well as 8 gynecological benign tumor patients were taken. The malignants were 5 squamous cervical carcinomas, 10 endometrial carcinomas and 17 epithelial ovarian cancers (EOC). The benign tumors were 4 ovarian epithelial tumors and 4 uterine myomas. Polymerase chain reaction-single strain conformation polymorphism (PCR-SSCP) and DNA sequencing were done to examine mtDNA mutation.
Results:
The mtDNA mutation rate and polymorphism rate were 68.8% and 56.3% respectively in 32 cases of malignants. The mtDNA mutation rate and polymorphism rate were 2/8 and 4/8 respectively in 8 cases of benigns. The difference between malignants and benigns was significant (P < 0.05). The hot point of mtDNA mutation were located in Cytb gene region. The characteristic mtDNA mutation is multigene and multisites mutation. The mojarity (63.6%) of patients involved in 2 more gene mutation.
Conclusions:
There is high frequent mtDNA coding area mutation in gynecological malignant tumors, indicating that mtDNA coding region mutation is closely related to gynecological malignants development and progression. It may be an important extra nuclear molecular genetic alteration in mechanisms of tumorigenesis.
Insights
Mitochondrial DNA (mtDNA) mutations are frequent in gynecological cancers, suggesting they play a role in tumor development. This study found significantly higher mutation rates in malignant tumors compared to benign ones.
Area of Science:
- Gynecologic Oncology
- Molecular Genetics
- Cancer Research
Context:
- Mitochondrial DNA (mtDNA) mutations are implicated in various cancers.
- Understanding mtDNA alterations in gynecologic tumors is crucial for identifying novel biomarkers and therapeutic targets.
Purpose:
- To investigate the prevalence and characteristics of mtDNA mutations in gynecologic tumor tissues.
- To explore the association between mtDNA mutations and tumorigenesis in gynecologic oncology.
Summary:
- This study analyzed tumor and normal tissues from 32 malignant and 8 benign gynecologic patients using PCR-SSCP and DNA sequencing.
- Malignant tumors exhibited a significantly higher mtDNA mutation rate (68.8%) and polymorphism rate (56.3%) compared to benign tumors (25% and 50%, respectively).
- Mutations were predominantly found in the Cytb gene region, often involving multiple genes and sites.
Impact:
- The findings highlight a high frequency of mtDNA coding area mutations in gynecologic malignant tumors.
- mtDNA coding region mutations are closely linked to the development and progression of gynecologic cancers.
- These mutations represent a significant extranuclear molecular genetic alteration in tumorigenesis mechanisms.
