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[Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells]
Shu-xun Hou1, Da-ming Sun, Gui-xin Du
1Department of Orthopeadics, 304th Hospital, People's Liberation Army, Beijing 100037, China.
Objectives:
To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.
Methods:
The BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.
Results:
PCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.
Conclusion:
Construction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.