Related Experiment Video
Updated: Jun 25, 2026

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii
Published on: April 16, 2016
Influence of precipitated calcium carbonate (CaCO3) on shiitake (Lentinula edodes) yield and mushroom size
Daniel J Royse1, Jose E Sanchez-Vazquez
1Department of Plant Pathology, Mushroom Research Center, 316 Buckhout Laboratory, Pennsylvania State University, University Park, PA 16802, USA. djr4@psu.edu
Abstract:
Synthetic substrate consisting of oak sawdust (50%), white millet (28%), winter rye (11%) and soft red wheat bran (11%) was non-supplemented or supplemented with 0.2%, 0.4% or 0.6% (dry weight basis) precipitated calcium carbonate (CaCO(3)). Shiitake (Lentinula edodes) was grown in two crops to determine the effect of three CaCO(3) levels on mushroom yield and size. Yields and biological efficiencies (averages for two crops) from substrates non-supplemented with CaCO(3) were lower by 14.1%, 18.4% and 24.9% compared to treatments supplemented with 0.2%, 0.4% and 0.6% CaCO(3), respectively. Mushroom size (weight) was larger with non-supplemented substrate (16.8 g) compared to substrate supplemented with 0.6% CaCO(3) (15.1 g). However, mushroom production was more consistent from crop to crop when 0.6% CaCO(3) was added to substrate.
Related Concept Videos
Factors Affecting Solubility
Factors Affecting Dissolution: Particle Size and Effective Surface Area
Fineness of Cement
Direct...
Carbonation Shrinkage
The concrete's permeability is slightly reduced as calcium carbonate produced during the reaction fills its pores. Furthermore, its strength is slightly enhanced as the water released during the reaction facilitates the...
Microbes in the Production of Fermented Foods
Production of Organic Acids

