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Tissue engineered cartilage generated from human trachea using DegraPol scaffold.

Lin Yang1, Stephan Korom, Manfred Welti

  • 1Division of Thoracic Surgery, Laboratory of Tissue Engineering, University Hospital, Raemistrasse 100, CH-8091 Zurich, Switzerland.

European Journal of Cardio-Thoracic Surgery : Official Journal of the European Association for Cardio-Thoracic Surgery
|August 5, 2003
PubMed
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Human tracheal chondrocytes (HTC) can be expanded in vitro and seeded onto DegraPol scaffolds to create cartilage-like tissue. Further research is needed to optimize culture conditions for complete cell infiltration and tissue formation in tracheal defect reconstruction.

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Reconstructing long segmental tracheal defects remains a significant clinical challenge with limited success.
  • Tissue engineering approaches using cartilage constructs have shown promise in animal studies.
  • The feasibility of using human tracheal chondrocytes (HTC) for fabricating tracheal replacements requires further investigation.

Purpose of the Study:

  • To optimize the isolation and cultivation of human tracheal cartilage.
  • To assess the feasibility of seeding HTC onto a novel 3-D polyester-urethane polymer (DegraPol).
  • To evaluate the potential of HTC-DegraPol constructs for tracheal tissue engineering.

Main Methods:

  • Human tracheal cartilage was harvested and chondrocytes were isolated and serially passaged.

Related Experiment Videos

  • Cells were expanded in vitro for 6-8 weeks, with some cultivated over agar.
  • Chondrocytes were seeded onto DegraPol scaffolds (150-200 µm pores) at a density of 2.4 x 10^7/ml for in vitro culture.

    • Main Results:

    • HTC demonstrated stable proliferation and expressed collagen type II in monolayer culture.
    • Seeding onto DegraPol supported continued cellular proliferation and extracellular matrix (ECM) production.
    • Alcian blue staining and scanning electron microscopy confirmed chondrocyte growth and ECM synthesis within the DegraPol scaffold, resembling native cartilage.

    Conclusions:

    • Harvested HTC expand well in vitro and can form new cartilage-like tissue on DegraPol.
    • The constructs show potential for engineering tracheal replacements.
    • Improved culture conditions are necessary to enhance cellular infiltration and growth throughout the cell-polymer constructs.