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Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis
Sunit Das1, Claudia Gerwin, Zu-Hang Sheng
1Synaptic Function Unit, NINDS, National Institutes of Health, Bethesda, Maryland 20892-4154, USA.
Abstract:
Syntaphilin is a brain-specific syntaxin-binding partner first characterized as an inhibitor of SNARE complex formation and neurotransmitter release. Here we show that syntaphilin also binds to dynamin-1 and through this interaction inhibits dynamin-mediated endocytosis. Immunoprecipitation studies from cross-linked rat synaptosomes demonstrate that an endogenous syntaphilin-dynamin-1 complex exists independently of dynamin-1 binding to amphiphysin. Functionally, syntaphilin expression inhibits transferrin internalization in COS-7 cells. These data reveal that syntaphilin is an inhibitor of both SNARE-based fusion and dynamin-mediated endocytosis.