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Updated: Sep 20, 2026

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Published on: April 16, 2021
Mutational analysis of cell wall biosynthesis in Mycobacterium avium
Jean-Pierre Laurent1, Kirsten Hauge, Kellie Burnside
1Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
Abstract:
The cell wall of the environmental pathogen Mycobacterium avium is important to its virulence and intrinsic antimicrobial resistance. To identify genes involved in cell wall biosynthesis, "transposome" insertion libraries were screened for mutants with altered colony morphology on medium containing the lipoprotein stain Congo red. Nineteen such mutants were isolated and mapped, including 10 with insertions in a functional island of cell wall biosynthetic genes that spans approximately 40 kb of the M. avium genome.
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