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Quantification of the c-myc gene in gastric carcinomas by the triplex polymerase chain reaction and high performance
S Yoshimura1, A Shishikura, S Koido
1Division of Molecular Life Science (Cell Biology), Tokai University School of Medicine, Kanagawa, Japan.
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
The triplex polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC) techniques were used to examine the state of amplification of the c-myc gene in gastric carcinomas. Sequences from the c-myc gene and from the two control genes were coamplified by PCR. The coamplified PCR products were separated and quantified by HPLC and the copy numbers of the c-myc gene were calculated by comparing the peak areas generated by PCR products. Increased copy numbers of the c-myc gene were found in 2 of 5 patients.