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Updated: Sep 20, 2026
Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders
Published on: September 4, 2011
Quantitative reverse transcriptase polymerase chain reaction analysis for KiSS-1 and orphan G-protein-coupled
Masahide Ikeguchi1, Yasuaki Hirooka, Nobuaki Kaibara
1Division of Operating Room, Faculty of Medicine, Tottori University, 36-1 Nishi-cho, 683-8504, Yonago, Japan. masaike@grape.med.tottori-u.ac.jp
Purpose:
KiSS-1 has been cloned as a human metastasis suppressor gene and an orphan G-protein-coupled receptor (hOT7T175) identified as the endogenous receptor of the KiSS-1 product. In the present study, we evaluated the clinical importance of KiSS-1 and hOT7T175 gene expression in hepatocellular carcinoma (HCC).
Methods:
The expression levels of KiSS-1, hOT7T175 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNAs (mRNAs) were analyzed quantitatively by real-time reverse transcriptase polymerase chain reaction (RT-PCR) in 60 surgically resected HCCs. The KiSS-1/GAPDH and hOT7T175/GAPDH ratios of tumors were compared with clinicopathological findings.
Results:
Loss of KiSS-1 mRNA expression was not detected in HCCs. The mean KiSS-1/GAPDH ratio did not change between non-cancerous cirrhotic livers and carcinomas. On the other hand, the average hOT7T175/GAPDH ratios increased from non-cancerous livers (0.08) to carcinomas (0.48). Overexpression of KiSS-1 and hOT7T175 genes was recognized in 6 tumors, which were in an advanced stage and showed poor survival.
Conclusion:
Overexpression of KiSS-1 and hOT7T175 genes was frequently observed and correlated with HCC progression; thus, the possibility that overexpressed KiSS-1 and hOT7T175 peptides mediate growth signals into cancer cells in HCCs is suggested.
Insights
KiSS-1 and its receptor hOT7T175 are overexpressed in hepatocellular carcinoma (HCC), correlating with advanced disease. This suggests these peptides may promote HCC growth, impacting patient survival.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- KiSS-1 is a human metastasis suppressor gene.
- hOT7T175 is an orphan G-protein-coupled receptor for the KiSS-1 product.
- Hepatocellular carcinoma (HCC) is a significant global health concern.
Purpose of the Study:
- To investigate the clinical significance of KiSS-1 and hOT7T175 gene expression in HCC.
- To correlate gene expression levels with clinicopathological features of HCC.
Main Methods:
- Quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to analyze KiSS-1 and hOT7T175 mRNA expression.
- Expression levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
- Analysis was performed on 60 surgically resected HCC samples and adjacent non-cancerous liver tissue.
Main Results:
- KiSS-1 mRNA expression loss was not observed in HCC.
- KiSS-1/GAPDH ratios showed no significant change between non-cancerous and cancerous liver tissues.
- hOT7T175/GAPDH ratios significantly increased from non-cancerous liver to HCC.
- Overexpression of both KiSS-1 and hOT7T175 was found in advanced-stage HCC with poor survival.
Conclusions:
- Overexpression of KiSS-1 and hOT7T175 is frequent in HCC and associated with disease progression.
- The findings suggest that overexpressed KiSS-1 and hOT7T175 peptides may mediate growth signals in HCC cells.
- Further research into the role of these peptides in HCC pathogenesis is warranted.
