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Syntheses of oligonucleotide derivatives with P(V) porphyrin and their properties
T Shimidzu1, H Segawa, M Kitamura
1Division of Molecular Engineering, Graduate School of Engineering, Kyoto University, Japan.
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
Two types of oligonucleotide derivatives which are substituted by P(V) porphyrin at the phosphorus atom of an internucleotidic linkage and at the 5'-terminal internucleotidic linkage via a spacer were synthesized (Fig. 1), and hybridization capabilities of them with complementary oligonucleotides were evaluated. A novel method for a sensing of oligonucleotide by the fluorescence quenching via photo-induced electron transfer between the P(V) porphyrin labeled oligonucleotide and pyrene-labeled one on the oligonucleotide template is reported.