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Related Experiment Videos

Tissue-based stem cells: ABC transporter proteins take centre stage.

Malcolm R Alison1

  • 1Department of Histopathology, Imperial College London at the Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.

The Journal of Pathology
|August 5, 2003
PubMed
Summary
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Identifying adult somatic stem cells is challenging due to a lack of specific markers. However, the side population (SP) phenotype, identified by Hoechst dye exclusion, shows promise as a universal stem cell marker.

Area of Science:

  • Stem cell biology
  • Cellular and molecular medicine
  • Tissue regeneration

Background:

  • Somatic stem cell identification in adult tissues relies heavily on location, lacking functional confirmation.
  • Isolation of live stem cells for functional assays has been hindered by the absence of specific cellular markers.

Purpose of the Study:

  • To investigate the potential of the side population (SP) phenotype as a universal marker for adult somatic stem cells.
  • To address the lack of functional data in identifying stem cells beyond the hematopoietic system.

Main Methods:

  • Utilized flow cytometry to identify and isolate cells based on their ability to exclude Hoechst dye.
  • Characterized the side population (SP) phenotype in various adult tissues.

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Main Results:

  • The side population (SP) phenotype, defined by Hoechst dye exclusion, was successfully identified.
  • This SP phenotype demonstrated potential as a marker for stem cells across different adult tissues, mirroring its use in hematopoietic stem cell isolation.

Conclusions:

  • The side population (SP) phenotype, identified via Hoechst dye exclusion, represents a promising universal marker for adult somatic stem cells.
  • This finding could facilitate the isolation and functional characterization of stem cells in various tissues, advancing regenerative medicine research.