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Updated: Sep 20, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Intramolecular dimers: a new design strategy for fluorescence-quenched probes
Mary Katherine Johansson1, Ronald M Cook
1Biosearch Technologies, 81 Digital Drive, Novato, CA 94949, USA. info@biosearchtech.com
Abstract:
Fluorogenic probes dual-labeled with reporter and quencher dyes use a change in fluorescence to monitor biochemical events (e.g., substrate binding or enzyme digestion). Such events change the reporter-quencher distance, which affects fluorescence. Recently, it is has been shown that static quenching through intramolecular dimers is an important mechanism that can sometimes be more efficient than Förster resonance energy transfer (FRET).
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