The effects of S-3-1 on lipid peroxidation and scavenging free radicals in vitro

S-3-1 is a simplified synthetic analogue of the active principle of Salvia miltiorrhioza. Electron spin resonance spectrometry using 5,5'-dimethyl-1-pyrroline-N-oxide as a scavenger of free radicals indicated that 200 microg/ml of S-3-1 scavenged 1,1-diphenyl-2-picrylhydrazyl radicals completely. 25 microg/ml of this compound quenched 100% of superoxide anion and a concentration of 250 microg/ml of S-3-1 quenched 63% of hydroxyl radicals. It was also shown that 3.56 mg/ml of S-3-1 could inhibit lipid peroxidation in microsome fraction from rat liver induced by FeSO4 and cysteine. These results show that S-3-1 is an effective antioxidant by scavenging free radicals.